浜哄师绾よ泲鐧�1(贵叠狈1)璇曞墏鐩�
浣跨敤璇存槑涔�
浜哄师绾よ泲鐧�1(贵叠狈1)璇曞墏鐩掑熀鏈師鐞�:
链瘯鍓傜洅閲囩敤鍙屾姉浣撳す蹇僂尝滨厂础娉曘赌傜敤鎶椾汉贵叠狈1鎶椾綋鍖呰浜庨叾鏍囨澘涓婏紝瀹為獙鏃舵牱鍝佹垨鏍囧噯鍝佷腑鐨勪汉贵叠狈1涓庡寘琚姉浣撶粨鍚堬紝娓哥鐨勬垚鍒呜娲楀幓銆备緷娆″姞鍏ョ敓鐗╃礌鍖栫殑鎶椾汉贵叠狈1鎶椾綋鍜岃荆鏍硅绷姘у寲鐗╅叾鏍囱鐨勪翰鍜岀礌銆傛姉浜篎叠狈1鎶椾綋涓庣粨鍚堝湪鍖呰鎶椾綋涓婄殑浜篎叠狈1缁撳悎銆佺敓鐗╃礌涓庝翰鍜岀礌鐗瑰紓镐х粨鍚堣赌屽舰鎴�**澶嶅悎鐗╋紝娓哥鐨勬垚鍒呜娲楀幓銆傚姞鍏ユ樉鑹插簳鐗�(罢惭叠)锛孴惭叠鍦ㄨ荆鏍硅绷姘у寲鐗╅叾鐨勫偓鍖栦笅锻堢幇钃濊壊锛屽姞缁堟娑插悗鍙樻垚榛勮壊銆傜敤閰舵爣浠湪450苍尘娉㈤暱澶勬祴翱顿鍊硷紝浜篎叠狈1娴撳害涓嶰顿450鍊间箣闂村憟姝ｆ瘮锛岄赌氳绷缁樺埗鏍囧噯鏇茬嚎璁＄畻鍑烘牱鍝佷腑浜篎叠狈1鐨勬禄搴︺赌�
浜哄师绾よ泲鐧�1(贵叠狈1)璇曞墏鐩掓弿杩�:
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鑻辨枃鍚嶇О
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Human FBN1 (Fibrillin 1) ELISA Kit
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涓枃鍚嶇О
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浜哄师绾よ泲鐧�1(贵叠狈1)閰惰仈**鍚搁檮娴嬪畾璇曞墏鐩�
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璐у彿
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X-EL-H2266c
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绉嶅睘
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贬耻尘补苍/浜�
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瑙勬牸
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96T/Kit (8*12 strips) 48T/Kit (8*6 strips)
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妫赌娴嬫柟娉�
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鍙屾姉浣撳す蹇冩硶
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妫赌娴嬭寖鍥�
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0.313~20ng/mL
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鐏垫晱搴�
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0.188ng/mL
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璇曞墏鐩掔粍鎴愶细
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涓枃鍚嶇О
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鑻辨枃鍚嶇О
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瑙勬牸
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淇濆瓨
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ELISA閰舵爣鏉匡纸鍙媶鍗革级
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Micro ELISA
Plate(Dismountable)
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8脳12 / 8脳6*
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4鈩�/-20鈩� #
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鍐诲共鏍囧噯鍝�
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Reference Standard
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2/1 鏀�*
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4鈩�/-20鈩� #
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鏍囧噯鍝�&鏍峰搧绋赌閲婃恫
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Reference Standard &
Sample Diluent
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1鐡� 20mL/12mL*
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4鈩�
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娴撶缉鐢熺墿绱犲寲鎶椾綋
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Concentrated Biotinylated
Detection Ab
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1鏀�&苍产蝉辫;120渭尝/70渭尝*
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4鈩�/-20鈩� #
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鐢熺墿绱犲寲鎶椾綋绋赌閲婃恫
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Biotinytated Detection Ab
Diluent
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1鐡� 10mL/6mL*
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4鈩�
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娴撶缉HRP閰剁粨鍚堢墿
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Concentrated HRP Conjugate
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1鏀�&苍产蝉辫;120渭尝/70渭尝*
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4鈩�(閬垮厜)
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閰剁粨鍚堢墿绋赌閲婃恫
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HRP Conjugate Diluent
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1鐡� 10mL/6mL*
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4鈩�
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娴撶缉娲楁钉娑诧纸25脳锛�
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ConcentratedWashBuffer
(25脳)
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1鐡� 30mL/16mL*
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4鈩�
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搴旷墿婧舵恫锛�TMB锛�
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Substrate Reagent
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1鐡� 10mL/6mL*
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4鈩�(閬垮厜)
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鍙嶅簲缁堟娑�
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Stop Solution
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1鐡� 10mL/6mL*
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4鈩�
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灏佹澘瑕呜啘
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Plate Sealer
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5/3 寮�*
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浜у搧璇存槑涔�
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Product Description
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1 浠�
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璐ㄦ鎶ュ憡
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Certificate of Analysis
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1 浠�
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鐗瑰埆璇存槑锛�
*: [96T/48T]锛堟墦寮赌鍖呰鍚庤鍙婃椂妫赌鏌ユ墍链夌墿鍝佹槸鍚﹂綈鍏ㄥ畬鏁达级
#: 涓赌锻ㄥ唴浣跨敤鍙瓨浜�4鈩冿紝闇赌闀挎椂闂村瓨鏀炬垨澶氭浣跨敤寤鸿瀛树簬-20鈩�.
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浜哄师绾よ泲鐧�1(贵叠狈1)璇曞墏鐩掓娴嬪墠鍑嗗宸ヤ綔:
1. 璇锋彁鍓�20鍒嗛挓浠庡啺绠变腑鍙栧嚭璇曞墏鐩掞紝骞宠 镊冲娓┿赌�
2. 灏嗘禄缂╂礂娑ゆ恫鐢ㄥ弻钂告按绋赌閲�(1:25)銆傛湭鐢ㄥ畬鐨勬斁鍥�4鈩冦赌备粠鍐扮涓彇鍑虹殑娴撶缉娲楁钉娑插彲鑳芥湁缁撴櫠锛屽睘浜庢宁哥幇璞★紝鍙敤40鈩冩按娴村井鍔犵儹浣跨粨鏅跺畬鍏ㄦ憾瑙ｅ悗鍐嶉厤鍒舵礂娑ゆ恫锛堝姞鐑俯搴︿笉瑕佽秴璞�50鈩冿紝浣跨敤鏃舵礂娑ゆ恫搴斾负瀹ゆ俯锛夈赌傚綋鏃ヤ娇鐢ㄣ赌�
3. 鏍囧噯鍝�: 浜�10000脳g绂诲绩1鍒嗛挓锛屽姞鍏ユ爣鍑嗗搧&鏍峰搧绋赌閲婃恫1.0mL镊冲喕骞叉爣鍑嗗搧涓紝鏃嬬揣绠＄洊锛岄润缃�10鍒嗛挓锛屼笂涓嬮鍊掓暟娆★紝寰呭叾鍏呭垎婧惰В鍚庯紝鐢ㄧЩ娑插櫒灏嗗叾杞昏交娣峰寑锛堟禄搴︿负8000pg/mL锛夈赌傜劧鍚庢牴鎹渶瑕佽繘琛屽赌嶆瘮绋赌閲婏纸娉细涓嶈鐩存帴鍦ㄥ弽搴斿瓟涓繘琛屽赌嶆瘮绋赌閲婏级銆傚缓璁厤鍒舵垚浠ヤ笅娴撳害锛氭寜鐓х█閲婃柟娉曞浘渚� 鏍囧噯鍝�&鏍峰搧绋赌閲婃恫鐩存帴浣滀负绌虹櫧瀛�0ng/mL銆傚閰嶅埗5ng/mL鏍囧噯鍝侊细鍙�0.5mL10ng/mL鐨勪笂杩版爣鍑嗗搧鍔犲叆鍚湁0.5mL鏍囧噯鍝�&鏍峰搧绋赌閲婃恫鐨�EP绠′腑锛屾贩鍖赌鍗冲彲锛屽叾浣欐禄搴︿緷姝ょ被鎺ㄣ赌�
4. 鐢熺墿绱犲寲鎶椾綋宸ヤ綔娑诧细瀹為獙鍓嶈绠楀綋娆″疄楠屾墍闇赌鐢ㄩ噺锛堜互100渭L/瀛旇锛夛紝瀹為檯閰嶅埗鏃跺簲澶氶厤鍒�100-200渭L銆备娇鐢ㄥ墠15鍒嗛挓锛屼互鐢熺墿绱犲寲鎶椾綋绋赌閲婃恫绋�閲婃祿缂╃敓鐗╃礌鍖栨姉浣�(1:100)鎴愬伐浣沧禄搴︺赌傚綋鏃ヤ娇鐢ㄣ赌�
5. 閰剁粨鍚堢墿宸ヤ綔娑诧细瀹為獙鍓嶈绠楀綋娆″疄楠屾墍闇赌鐢ㄩ噺锛堜互100渭L/瀛旇锛夛紝瀹為檯閰嶅埗鏃跺簲澶氶厤鍒�100-200渭L銆备娇鐢ㄥ墠15鍒嗛挓锛屼互閰剁粨鍚堢墿绋赌閲婃恫绋�閲婃祿缂�HRP閰剁粨鍚堢墿(1:100)鎴愬伐浣沧禄搴︺赌�
褰撴棩浣跨敤銆�
浜哄师绾よ泲鐧�1(贵叠狈1)璇曞墏鐩�鏍囧噯鍝佺█閲婃柟娉曞浘渚嬶细锛堜互500渭L/绠′负渚嬶紝涔熷彲鏍规嵁瀹為檯鐢ㄩ噺鏉ョ█閲婏紝濡�200渭L/绠★级
1. 鍦ㄥ悇瀛斾腑鍔犲叆鏍囧噯鍝佹垨鏍峰搧鍚�100渭L锛� 37鈩冨镶�90鍒嗛挓
2. 鍔犲叆100渭L 鐢熺墿绱犲寲鎶椾綋宸ヤ綔娑诧紝37鈩冨镶�60鍒嗛挓
3. 娲楁钉3娆�
4. 鍔犲叆100渭L閰剁粨鍚堢墿宸ヤ綔娑诧紝 37鈩冨镶�30鍒嗛挓
5. 娲楁钉5娆�
6. 鍔犲叆90渭L搴旷墿婧舵恫锛� 37鈩冨镶�15鍒嗛挓宸﹀彸
7. 鍔犲叆50渭L缁堟娑诧紝绔嬪嵆鍦�450nm娉㈤暱澶勬祴閲�OD鍊�
8. 缁撴灉璁＄畻
涓赌銆佹搷浣滃洜绱犲ELISA缁撴灉鐨勫奖鍝�ELISA鎿崭綔姝ラ澶嶆潅锛屾搷浣滀笉褰撳皢寮曡捣杈冨ぇ鐨勮宸赌备互涓嬪彊杩板悇涓楠や腑鐨勫奖鍝嶅洜绱犮赌�
1.鍔犳牱
2.娓╄偛
3.娲楁钉
4.鏄捐壊
5.姣旇壊
浜屻赌佺伆鍖虹殑璁剧疆閫氬父鎯呭喌涓嬶紝ELISA瀹氭赌у疄楠屼互鈥滈槼镐р赌濆拰鈥滈槾镐р赌濇潵鎶ュ憡缁撴灉锛屼袱钥呴棿链変竴鏉″垎鐣岀嚎琚О涓衡赌滈槼镐у垽鏂赌尖赌濓纸cut-off value锛�CO鍊硷级锛岃繖鏄畾镐�**娴嬪畾缁撴灉鎶ュ憡鐨勪緷鎹赌�
涓夈赌佹爣链鏌�ELISA鎵嬪伐妫赌娴嬭绷绋嬪鏉傦紝褰卞搷鍥犵礌杈冨锛屽嵆浣垮鍐呰川鎺у湪鎺э紝涔熷彲鑳藉洜瀛旈棿宸紓钥岄赌犳垚缁撴灉鐨勫樊寮傦紝鍥犳鍔犲ぇ澶嶆煡鍔涘害鏄繚璇佺粨鏋滃噯纭赌х殑鍙潬鏂规硶锛屾瘮濡傚HBsAg銆�HBeAg銆�HCV-Ab銆�HIV-Ab銆�TP-Ab绛夐」鐩殑鍙枒銆佸急闃虫赌ф爣链强灏戣妯″纺鐨勬娴嬬粨鏋滆繘琛屽鏌ャ赌�
鍥涖赌佺粨鏋滃垽鏂拰鎶ュ憡宁哥敤涓嬪垪鍑犵鏂规硶琛ㄧず缁撴灉锛�
1.瀹氭赌ф祴瀹�
2.鍗婂畾閲忔祴瀹� 缁撴灉涓赌鑸互婊村害琛ㄧず銆�
3.瀹氶噺娴嬪畾 鍗崇敤宸茬煡閲忕殑鏍囧噯鍝佷綔涓赌绯诲垪绋赌閲婂悗杩涜ELISA娴嬪畾锛岀粯鍒舵爣鍑嗘洸绾匡紝缁撴灉浠�**閲忔垨鍗曚綅琛ㄧず銆傚湪ELISA瀹氶噺妫赌娴嬩腑姣忎竴鍧楀弽搴旀澘閮藉繀椤荤粯鍒剁浉搴旂殑鏍囧噯鏇茬嚎銆�
娉ㄦ剰浜嬮」锛�
A 浠旂粏闃呰璇存槑涔︺赌�
B 妫赌鏌ヨ瘯鍓傜洅鏍囩涓婄殑链夋晥链熴赌傚鏋滆秴璞伶湁鏁堟湡锛岃鍕夸娇鐢ㄣ赌�
C 鎸夎鏄庝功纭畾鎵赌链夎瘯鍓傞綈鍏纸鏁伴噺銆佷綋绉级銆�
D 鏍囨湰鍒跺瑕佽锣冿紝姣忎唤鏍囨湰浣撶Н瑕佹寜2-3涓瀛斾互涓婄殑閲忓埗澶囷纸璐瓨锛夛紝灏介噺鍒呜锅氬浠姐赌傜煭链熷唴鏃犳硶瀹為獙钥咃紝娉ㄦ剰浣庢俯淇濆瓨銆�
E 鍑嗗濂芥墍链夊疄楠岄澶栨墍闇赌鐗╁搧锛岋纸姣斿绉绘恫鍣紝璇旷锛屾竻娲楀櫒锛岄叾鏍囦华锛夈赌�
F 鎸夎鏄庝功灏嗘墍鐢ㄨ瘯鍓傚钩琛¤嚦瀹ゆ俯锛屾牴鎹娴嬫爣链暟閲忕‘瀹氭墍闇赌璇曞墏鐨勯噺銆�
G 妫赌鏌ヨ瘯鍓傜洅鍐呮疮绉嶈瘯鍓傜殑璐瓨鏉′欢锛屼繚璇佹墍链夎瘯鍓傚潎鎸夌収璇曞墏鐩掑唴璇存槑涔︽帹钻愮殑鏉′欢瀛樺偍銆�
H 妫赌鏌ヤ笉绋冲畾鎴栬赌呭彉璐ㄧ殑璇曞墏婧舵恫锛堟瘮濡傛矇娣赌鎴栧彉鑹诧级銆傛湁浜涜瘯鍓傦紝姣斿鏍囧噯鍝佺█閲婃恫鎴栬赌呮娴嬬█閲婃恫锛屽彲鑳藉湪璁捐鏃跺氨鍚湁娌夋稳鐗┿赌傛墍链夎瘯鍓傚湪婊村叆閰舵爣鏉夸箣鍓嶏紝蹇呴渶鍏呭垎娣峰寑銆傝赌岀敱浜庢矇娣赌鐗╃殑鐗规赌э紝鍦ㄥ姞鍏ラ叾鏍囨澘涔嬪墠锛屽繀闇赌涓嶅仠鎼呮妈銆�
I 淇濊瘉鍏呭垎鐨勫镶叉椂闂村拰娓╁害銆�
J 鍒囧嬁浣跨敤涓嶅悓鐢熶骇鎵瑰彿鐨勮瘯鍓傚彇浠ｇ幇链夎瘯鍓傛垨娣峰悎鐜版湁璇曞墏銆�
K 鍦ㄦ贩鍚堟垨婧惰В铔嬬櫧婧舵恫鏃讹紝閬垮厤娉℃搏浜х敓銆�
L 鍦ㄥ疄楠屽紑濮嬩箣鍓嶏紝瀹夋帓濂藉疄楠屾祦绋嬨赌�
M 鍦ㄥ疄楠屽紑濮嬩箣鍓嶏紝娓呮磥宸ヤ綔鍙般赌�
N 鑻ユ湁闂锛屽簲涓庢垜鍏徃鎴栦唬鐞嗗晢鐨勬妧链敮鎸佽仈绯�
浜哄师绾よ泲鐧�1(贵叠狈1)璇曞墏鐩掔粨鏋滃垽鏂�:
1. 姣忎釜鏍囧噯鍝佺殑OD鍊煎噺铡荤┖鐧藉瓟鐨�OD鍊煎悗浣滃浘锛屽璁剧疆澶嶅瓟锛屽垯搴斿彇鍏跺钩鍧囧赌艰绠椼赌备互鏍囧噯鍝佺殑娴撳害涓烘í鍧愭爣锛�OD鍊间负绾靛潗鏍囷紝缁樺嚭鏍囧噯鏇茬嚎銆备害鍙互OD鍊间负妯潗鏍囷紝鏍囧噯鍝佺殑娴撳害涓虹旱鍧愭爣锛岀粯鍑烘爣鍑嗘洸绾裤赌�
2. 鎺ㄨ崘浣跨敤涓扑笟鐨勬洸绾垮埗浣滆蒋浠讹紝濡�curve expert 1.3鎴�1.4锛屽湪杞欢鐣岄溃鏃㈠彲鏍规嵁鏍峰搧OD鍊硷紝鐢辨爣鍑嗘洸绾挎煡鍑虹浉搴旂殑娴撳害锛屼箻浠ョ█閲婂赌嶆暟锛涗害鍙皢鏍峰搧鐨�OD鍊间唬鍏ユ爣鍑嗘洸绾跨殑鎷熷悎鏂圭▼寮忥紝璁＄畻鍑烘牱鍝佹禄搴︼紝鍐崭箻浠ョ█閲婂赌嶆暟锛屽嵆涓烘牱鍝佺殑瀹為檯娴撳害銆�
3. 鑻ユ牱鍝�OD鍊奸珮浜庢爣鍑嗘洸绾夸笂闄愶紝搴旈赌傚綋绋赌閲婂悗閲嶆祴锛岃绠楁禄搴︽椂搴斾箻浠ョ█閲婂赌嶆暟銆�
浜�铡熺氦铔嬬櫧1(贵叠狈1)璇曞墏鐩�链夋晥链熺ǔ瀹氭赌ц赌冨疗
链瘯楠屼互铡熺氦铔嬬櫧1(贵叠狈1)涓轰緥
1. OD鍊硷细
姝ｅ父淇濆瓨鏉′欢涓嬫湁鏁堟湡鍐呭拰瓒呰绷链夋晥链�2涓湀鎵赌娴�OD鍊兼瘮杈�
姝ｅ父链夋晥链熷唴OD鍊硷细 3.251锛�2.845锛�2.126, 1.859, 1.463, 1.022, 0.710,
0.08
瓒呰绷2涓湀链夋晥链�OD鍊硷细3.1.7, 2.643, 2.125, 1.796, 1.453, 0.876, 0.432, 0.07
2.鍥炴敹鐜囷細鍒嗗埆浜庡畾鍊艰《逆竻鍙婅娴嗕腑鍔犲叆涓�瀹氶噺鐨勫師绾よ泲鐧�1(FBN1)鏍囧噯鍝侊紝閲嶅娴嬪畾骞惰绠楀叾鍝佸潎鍊硷紝鍥炴敹鐜囦负娴嬪畾鍊间笌鐞嗚鍊肩殑姣旂巼銆�
姝ｅ父淇濆瓨鏉′欢涓嬫湁鏁堟湡鍐呭拰杩囨湁鏁堟湡2涓湀elisa 璇曞墏鐩掑洖鏀剁巼妫赌娴嬬粨鏋�
|
Sample
|
Conditions
|
Recovery range(%)
|
Average(%)
|
|
Serum(n=6)
|
normal
|
88-106
|
95
|
|
After 12 month
|
80-100
|
87
|
|
EDTA plasma(n=6)
|
normal
|
88-106
|
97
|
|
After 12 month
|
78-98
|
87
|
3. 绾挎�э細鍒嗗埆浜庡畾鍊艰《逆竻鍙婅娴嗕腑鍔犲叆涓�瀹氶噺鐨勫師绾よ泲鐧�1(FBN1)鏍囧噯鍝侊紝骞跺皢鏍囨湰鎸�1:2,1:4,1:8绋赌閲婏紝绾挎赌ц寖鍥村嵆涓虹█閲婂悗鏍锋湰涓师绾よ泲鐧�1(贵叠狈1)鍚噺鐨勬祴瀹氬赌间笌鐞呜鍊肩殑姣旂巼銆�
姝ｅ父淇濆瓨鏉′欢涓嬫湁鏁堟湡鍐呭拰杩囨湁鏁堟湡2涓湀elisa 璇曞墏鐩掑洖绾挎赌ф娴嬬粨鏋�
|
Sample
|
Conditions
|
1:2
|
1:4
|
1:8
|
|
Serum(n=6)
|
normal
|
81-92%
|
85-90%
|
89-105%
|
|
After 12month
|
80-88%
|
82-87%
|
87-101%
|
|
EDTA plasma(n=6)
|
normal
|
93-104%
|
81-97%
|
91-96%
|
|
After 12month
|
82-95%
|
75-82%
|
74-90%
|
4. 绮惧瘑搴︼细浠ユ牱鍝佹祴瀹氬赌肩殑鍙樺紓绯绘暟CV琛ㄧず銆�CV锛�%锛�=SD/meanx100
鎵瑰唴宸细鍙栧悓涓赌鎵规璇曞墏鐩掑浣庡赌硷紝涓赌煎拰楂樺赌兼牱链繘琛屽畾閲忔娴嬶紝姣忎唤鏍锋湰娴嬪畾20娆★紝鍒嗗埆璁＄畻涓嶅悓娴撳害鏍锋湰鐨勫钩鍧囧赌煎拰SD鍊笺赌�
鎵归棿宸细閫夊彇 3 涓笉鍚屾壒娆＄殑璇曞墏鐩掑垎鍒浣庛赌佷腑銆侀珮鍊煎畾鍊兼牱链繘琛屽畾閲忔祴瀹氾紝 姣忎釜鏍锋湰浣跨敤鍚屼竴璇曞墏鐩掗吨澶嶆祴瀹� 8 娆★紝鍒嗗埆璁＄畻涓嶅悓娴撳害鏍锋湰鐨勫钩鍧囧赌煎强 SD 鍊笺赌�(鎵瑰唴宸细 CV<10%;鎵归棿宸细 CV<11%)
姝ｅ父淇濆瓨鏉′欢涓嬫湁鏁堟湡鍐呭拰杩囨湁鏁堟湡2涓湀elisa 璇曞墏鐩掑洖绮惧瘑搴︽娴嬬粨鏋�
Intra-assay precision
|
Conditions
|
normal
|
After 12month
|
normal
|
After 12month
|
normal
|
After 12month
|
|
sample
|
1
|
1
|
2
|
2
|
3
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean(ng/ml)
|
16.68
|
14.22
|
70.25
|
62.36
|
369.75
|
327.88
|
|
SD
|
1.08
|
1.25
|
4.21
|
5.31
|
24.69
|
32.87
|
|
CV(%)
|
6.5
|
8.8
|
6.0
|
8.5
|
6.6
|
10
|
|
Conditions
|
normal
|
After 12month
|
normal
|
After 12month
|
normal
|
After 12month
|
|
Sample
|
1
|
1
|
2
|
2
|
3
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean(ng/ml)
|
18.72
|
13.68
|
65.98
|
61.25
|
387.22
|
425.26
|
|
SD
|
1.45
|
1.52
|
4.83
|
5.18
|
28.49
|
36.87
|
|
CV锛�%锛�
|
7.7
|
11.1
|
7.3
|
8.5
|
7.3
|
8.7
|
浜哄师绾よ泲鐧�1(贵叠狈1)璇曞墏鐩掍細鍑虹幇鐨勯棶棰樺強瑙ｅ喅鍔炴硶锛�
1. 鍔犲叆鍙嶅簲缁堟娑插悗锛屾病链夊惛鍏夊赌兼垨鍚稿厜鍊煎亸浣庛赌�
a.铡熷洜锛氭湭鍔犲叆閰舵爣璁扮墿銆�
瑙ｅ喅鍔炴硶锛氳鎸夌収鎿崭綔姝ラ杩涜銆�
b.铡熷洜锛氳瘯鍓傜洅鍐呭鐗╂湭鑳藉厖鍒嗗洖銆�
瑙ｅ喅鍔炴硶锛氱‘瀹氳瘯鍓傜洅鍐呭鐗╁洖娓╁悗鍐嶈繘琛屾搷浣溿赌�
c.铡熷洜锛氬娓╁お浣庛赌�
瑙ｅ喅鍔炴硶锛氳嫢瀹ゆ俯澶綆(<19鈩�)锛岃浜庢搷浣沧楠�4锛岄赌傚綋寤堕暱娓╄偛鏃堕棿銆�
d.铡熷洜锛氭湭浣跨敤璇曞墏鐩掔殑娓呮礂娑叉竻娲椼赌�
瑙ｅ喅鍔炴硶锛� 璇风敤璇曞墏鐩掑唴鎵赌鎻愪緵鐨勬竻娲楁恫娓呮礂銆�
e.铡熷洜锛氳瘯鍓傜洅宸茶绷链夋晥链熼檺銆�
瑙ｅ喅鍔炴硶锛氳鏇存崲鎴愪粛鍦ㄦ湁鏁堟湡闄愬唴鐨勮瘯鍓傜洅銆�
2. 鏍囧噯鏇茬嚎绾挎赌т笉浣筹紝鎴栨槸骞宠镐т笉濂姐赌�
a.铡熷洜锛氭俯镶蹭綅缃伩鍏変笉濂芥垨鍙楀埌姘旀祦骞叉壈銆�
瑙ｅ喅鍔炴硶锛� 璇风‘璁ゆ俯镶蹭綅缃棦涓嶉赌忓厜涔熶笉閫忛(濡傛娊灞夊唴)銆�
b.铡熷洜锛氭搷浣沧椂闂存嫋澶箙銆�
瑙ｅ喅鍔炴硶锛氳鍦ㄦ柟娉旷啛缁冨悗鍐嶈繘琛屾搷浣溿赌�
c.铡熷洜锛氬井瀛旈棿鐩镐簰姹℃煋銆�
瑙ｅ喅鍔炴硶锛� 鍔犳牱鍝佹椂锛岃灏忓绩鍕挎簠鍑哄井瀛斿锛屼互鍏嶉赌犳垚鍏跺畠寰瓟鐨勬薄鏌撱赌�
d.铡熷洜锛氭爣鍑嗗搧鎴栭叾鏍囱鐗╂墍鍔犲叆鐨勯噺涓崭竴镊淬赌�
瑙ｅ喅鍔炴硶锛氳浣跨敤宸叉牎姝ｈ绷鐨勭Щ娑叉灙锛屽苟纭繚鍏朵笌鍚稿ご涔嬮棿链夐珮搴﹀瘑鍚堟赌с赌�
e.铡熷洜锛氭坊鍔犳牱鍝佹垨璇曞墏鐨勬搷浣沧柟娉曚笉姝ｇ‘銆�
瑙ｅ喅鍔炴硶锛氬姞鏍峰搧鎴栬瘯鍓傛椂锛屽惛澶磋鍕挎帴瑙﹀井瀛斻赌�
f.铡熷洜锛氭礂鏉胯绷绋嬪彂鐢熼棶棰�(濡傜璺牭濉炪赌佹礂瀹屾澘鍚庢湭绔嬪埢杩涜涓嬩竴姝ラ)銆�
瑙ｅ喅鍔炴硶锛氳闅忔椂鐩戞带娲楁澘链烘礂鏉胯绷绋嬶紝娲楀畬鍚庣珛鍗宠繘琛屼笅涓赌姝ラ銆�
3. 鍚稿厜鍊煎潎锅忛珮(鎴栫嚎镐т笉澶熼櫋)銆�
a.铡熷洜锛氬娓╁お楂�(>25鈩�)銆�
瑙ｅ喅鍔炴硶锛� 鑻ユ棤娉曢檷浣庡鍐呮俯搴︼紝璇烽赌傚綋缂╃煭姝ラ4鐨勬俯镶叉椂闂淬赌�
b.铡熷洜锛氬簳鐗╂憾娑插弹鍒版薄鏌撱赌�
瑙ｅ喅鍔炴硶锛� 鑻ュ彂鐜板簳鐗╂憾娑插凡锻堢幇娣¤摑鑹诧紝璇蜂笉瑕佸啀浣跨敤銆�
c.鍘熷洜锛氬弽搴旀椂闂磋秴璞铃お涔呫��
瑙ｅ喅鍔炴硶锛氳鎺у埗鍙嶅簲鏃堕棿銆�
d.铡熷洜锛氶叾鏍囱鐗╂薄鏌扑简鐩掑唴鍏跺畠璇曞墏銆�
瑙ｅ喅鍔炴硶锛氢娇鐢ㄨ绷鐨勫惛澶村簲绔嬪嵆涓㈠纯锛屽彲閬垮厤璇曞墏闂寸浉浜掓薄鏌掳紝鍑℃槸璇曞墏(鐗瑰埆鏄叾鏍囱鐗╀笌搴旷墿婧舵恫)鎺ヨЕ杩囩殑瀹瑰櫒搴旂珛鍗虫竻娲楀共鍑赌銆�(鍏堜互婕傜櫧姘存蹈娉★紝鍐崭互娓呮按鍐叉礂骞插噣锛屽彲纭繚鏃犳画鐣�)
4. 闃存�ф爣鍑嗗搧鐨勫惛鍏夊�间綆浜庨槼鎬ф爣鍑嗗搧鐨勫惛鍏夊�笺赌�
a.铡熷洜锛氬井瀛斾腑鐨勮瘯鍓傛湭鑳藉厖鍒嗘贩鍚堛赌�
瑙ｅ喅鍔炴硶锛� 璇曞墏鍔犲畬鍚庯紝闇赌杞绘暡鐩樺洓锻ㄤ娇鍏跺厖鍒嗘贩鍚堝潎鍖赌銆�
b.铡熷洜锛氭礂鏉胯绷绋嬪彂鐢熼棶棰�(鍚�2-f.)銆�
瑙ｅ喅鍔炴硶锛氳鍙傝赌�2-f.
c.铡熷洜锛氭坊鍔犳牱鍝佹垨閰舵爣璁扮墿鐨勯噺涓崭竴镊淬赌�
瑙ｅ喅鍔炴硶锛� 璇峰弬钥�2-d.