磷酸化丝氨酸/苏氨酸蛋白激酶惭厂罢4,惭厂罢3,厂罢碍25抗体
规格：1尘驳/1尘濒
英文名： phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174)
别名： phospho-MST4+MST3+STK25(phospho T178 + T190 + T174); p-MST4 + MST3 + STK25 (phospho T178 + T190 + T174); Mammalian STE20 like protein kinase 3; Mammalian Ste20 like protein kinase 4; Mammalian sterile
分子量： 48kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthesised phosphopeptide derived from human
交叉反应：Human, Mouse, Rat, Cow, Horse, Sheep,
细胞定位：细胞核 细胞浆 细胞膜
磷酸化丝氨酸/苏氨酸蛋白激酶惭厂罢4,惭厂罢3,厂罢碍25抗体产物介绍：background: Novel human Ste20-related kinase Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation. It is pro apoptotic and is highly expressed in placenta, thymus, and peripheral blood leukocytes. Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. This may also be activated by C terminal cleavage. MST3 or Mammalian Sterile 20-like kinase 3 is a member of the germinal center kinase-III family. MST3 contains a conserved kinase domain at its NH(2)-terminus and a regulatory domain at its COOH-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation. Expression of COOH-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis磷酸化丝氨酸/苏氨酸蛋白激酶惭厂罢4,惭厂罢3,厂罢碍25抗体. It can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST. Mitogen activated protein kinase cascades have been conserved throughout evolution. In mammals, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines. In yeast, STK25 functions upstream of the MAPK cascade. Function: Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve. Subunit: Monomer. Subcellular Location: STK25, MST4: Cytoplasmic, Golgi Apparatus. MST3: Cytoplasm. Nucleus. Membrane. Note=The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane. Tissue Specificity: MST3: Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex. Post-translational modifications: MST3: Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.磷酸化丝氨酸/苏氨酸蛋白激酶惭厂罢4,惭厂罢3,厂罢碍25抗体 Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. Gene ID: 10494 Database links: Entrez Gene: 10494 Human Entrez Gene: 51765 Human Entrez Gene: 8428 Human Omim: 300547 Human Omim: 602255 Human Omim: 604984 Human SwissProt: O00506 Human SwissProt: Q9P289 Human SwissProt: Q9Y6E0 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化丝氨酸/苏氨酸蛋白激酶惭厂罢4,惭厂罢3,厂罢碍25抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 细胞凋亡 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid