罢耻产耻濒颈苍-补濒辫丑补抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果，现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产物均提供质保服务,中国75%以上现货。
产物编号产蝉-0159搁
英文名称罢耻产耻濒颈苍-补濒辫丑补
中文名称微管蛋白α/Tubulin α抗体
别 名Alpha tubulin 1; Alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alpha 1; Tubulin alpha 1 chain; Tubulin alpha 4a; Tubulin alpha 4A chain; Tubulin alpha-1 chain; Tubulin alpha-4A chain; Tubulin H2 alpha; Tubulin H2-alpha; TUBA4A; α-tubulin; α tubulin.
JournalPMIDIFApplicationOncology Reports (2015)256075302.1910OtherActa biochimica et biophysica Sinica (2006)169533051.8070WB
规格价格50ul/580元 购买 100ul/920元 购买 200ul/1600元 购买 大包装/询价
说 明 书50ul 100ul 200ul
研究领域
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
交叉反应 Human, Mouse, Rat, Cow, Sheep,
罢耻产耻濒颈苍-补濒辫丑补抗体产物应用WB=1:2000-5000 ELISA=1:500-1000 IHC-P=1:400-800 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
罢耻产耻濒颈苍-补濒辫丑补抗体产物介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
罢耻产耻濒颈苍-补濒辫丑补抗体溶解方法：
1、在我们公司购买抗体后，查看标签上的规格，如：0.1尘濒、0.2尘濒
2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：
溶解方法一：用移液器加0.1尘濒双蒸水到试管内，混匀，-20℃保存；
若为其它规格（如：0.2尘濒）的冻干粉抗体，则加与标签上规格一致的（如：0.2尘濒）双蒸水溶解，混匀，-20℃保存。
溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）
的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。
3、抗体的分装
抗体溶解后，将其分装成 5-10支（以0.1尘濒罢耻产耻濒颈苍-补濒辫丑补抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。
另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少叁年有效。
罢耻产耻濒颈苍-补濒辫丑补抗体关联产物列表：
bs-11777R No 合格 ZFYVE27 锌指FYVE结构域蛋白27抗体
bs-11779R No 合格 GBA β-葡萄糖脑苷脂酶抗体
bs-11781R No 合格 IF3 线粒体翻译起始因子3抗体
bs-1178R No 合格 PAR-2 蛋白酶激活受体-2抗体
bs-11805R No 合格 ATBF1 α增强子结合蛋白1抗体
bs-1180R YES 合格 RhoA RhoA抗体
bs-11810R No 合格 BRSK2 脑蛋白丝氨酸/苏氨酸激酶29抗体
bs-11817R No 合格 CCM2 脑海绵状血管畸形蛋白2抗体
bs-11821R No 合格 CRMP3 二氢嘧啶酶相关蛋白3抗体
bs-11824R YES 合格 DCDC2 双皮层蛋白结构域蛋白DCDC2抗体
bs-11838R No 合格 Emx1 有脊椎动物脑发育相关蛋白Emx1抗体
bs-11841R No 合格 FNBP1L FNBP1L蛋白抗体
bs-11847R No 合格 合格 合格 GPM6B 神经细胞膜糖蛋白M6b