pSP73 - 91蜜桃视频

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0094	pSP73	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	T7
复制子:	ColE1 ori
质粒分类:	大肠杆菌载体；克隆质粒
质粒大小:	2464bp
原核抗性:	氨苄青霉素础尘辫
克隆菌株:	顿贬5α
培养条件:	37℃，有氧，尝叠
5'测序引物:	Sp6:ATTTAGGTGACACTATAGAA
3'测序引物:	T7:TAATACGACTCACTATAGGG
备注:	丰富的酶切位点

质粒介绍

The pSP73 Vector offers a wide range of restriction sites, providing greater versatility in cloning and transcription of RNA in vitro. The pSP73 Vector contains the SP6 and T7 RNA polymerase promoters and a unique multiple cloning region, which includes restriction sites for BglII, EcoRV, ClaI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII, PvuII and XhoI. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning region.

质粒图谱

质粒序列

LOCUS       Exported                2464 bp ds-DNA    circular SYN 28-9-2015
DEFINITION .
ACCESSION   .
VERSION     .
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 2464)
AUTHORS   admin
TITLE     Direct Submission
JOURNAL   Exported 2015-9-28 from MLCC
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    24..80
                     /gene="
                     "
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(102..120)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(378..966)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1137..1997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1998..2102)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        2448..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaaccagatc tgatatcatc gatgaattcg agctcggtac ccggggatcc tctagagtcg
       61 acctgcaggc atgcaagctt cagctgctcg aggccggtct ccctatagtg agtcgtatta
      121 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      181 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      541 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
      601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      781 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
      841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2101 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2161 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     2221 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     2281 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     2341 aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
     2401 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     2461 tata
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