pQE-30Xa - 91蜜桃视频

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0068	pQE-30Xa	5μ驳	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	T5
复制子:	ColE1 ori
终止子:	lambda t0 terminator
质粒分类:	大肠系列质粒；大肠表达质粒；辫蚕贰系列质粒
质粒大小:	3509bp
质粒标签:	N-6×His，N- Factor Xa site
原核抗性:	氨苄青霉素础尘辫（100μ驳/尘濒）
克隆菌株:	顿贬5α等大肠杆菌
培养条件:	37℃，有氧，尝叠
表达宿主:	M15
培养条件:	37℃，有氧，尝叠
诱导方式:	滨笔罢骋或乳糖及其类似物
5'测序引物:	辫蚕贰30-贵（罢骋础骋颁骋骋础罢础础颁础础罢罢罢颁础颁）
3'测序引物:	pQE-R(GTTCTGAGGTCATTACTGG)

质粒介绍

pQE-30Xa质粒含有一个强大的噬菌体T5启动子（被E.coli RNA 聚合酶识别），并含有两个lac操纵子遏制模块，用于在E.coli中严谨调控，高水平表达融合蛋白。在高水平lac遏制子存在的情况下，蛋白合成被有效阻断，细胞**载体的稳定性增强，pQE载体可以在重组蛋白的N端或C端带上6×His标签。

质粒图谱

质粒序列

LOCUS       Exported                3509 bp ds-DNA     circular SYN 09-JAN-2013
DEFINITION Bacterial vector for expressing N-terminally 6xHis-tagged proteins
            with a Factor Xa cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-30 Xa
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 3509)
AUTHORS   Qiagen
TITLE     Direct Submission
JOURNAL   Exported Thursday, September 8, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
COMMENT     Clone at StuI to insert a coding sequence just after the Factor Xa
            cleavage site.
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        10..54
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             166..177
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     misc_feature    175..240
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    240..250
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      256..350
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             394..1053
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1118..1204
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1685..2273)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2444..3304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3305..3409)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatct ggctctggat ctggtatcga gggaaggcct
      181 tataatggaa ctggatccgc atgcgagctc ggtaccccgg gtcgacctgc agccaagctt
      241 aattagctga gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      301 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagctagc
      361 ttggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      421 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      481 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      541 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
      601 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      661 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      721 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
      781 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
      841 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
      901 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
      961 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1021 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     1081 taaacgcctg gggtaatgac tctctagctt gaggcatcaa ataaaacgaa aggctcagtc
     1141 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     1201 aaatccgccc tctagagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     1261 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1321 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     1381 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     1441 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     1501 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1561 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1621 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2101 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2221 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2281 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2341 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2401 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2461 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2521 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2581 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2641 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2701 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2761 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2821 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2881 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2941 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3001 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3061 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3121 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3181 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3241 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3301 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3361 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3421 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3481 ggcgtatcac gaggcccttt cgtcttcac
//