pQE-31

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0069	pQE-31	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

复制子:	Ori
终止子:	Lambda t0
质粒分类:	大肠杆菌载体；辫蚕贰系列表达质粒
质粒大小:	3463bp
质粒标签:	N-His
原核抗性:	氨苄青霉素础尘辫
克隆菌株:	顿贬5α
培养条件:	37℃，有氧，尝叠
表达宿主:	M15
培养条件:	37℃，有氧，尝叠
诱导方式:	无需诱导
5'测序引物:	pQE30-F: AGCGGATAACAATTTCACACAG
3'测序引物:	pQE30-R: TTCTGAGGTCATTACTGGATC

质粒介绍

质粒图谱

质粒序列

LOCUS       Exported                3463 bp ds-DNA     circular SYN 08-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    PQE-31
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 3463)
AUTHORS   .
TITLE     Direct Submission
JOURNAL   Exported Thursday, September 8, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..54
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      210..304
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             348..1007
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1072..1158
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1313..1453
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1639..2227)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2398..3258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3259..3363)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatctcacc atcaccatca ccatacggat ccgcatgcga gctcggtacc ccgggtcgac
      181 ctgcagccaa gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc
      241 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
      301 gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      361 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      421 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      481 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      541 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
      601 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
      661 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
      721 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
      781 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
      841 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
      901 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
      961 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     1021 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
     1081 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1141 ctcctgagta ggacaaatcc gccctctaga gctgcctcgc gcgtttcggt gatgacggtg
     1201 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1261 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1321 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1381 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     1441 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1501 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1561 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1621 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1741 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1801 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1861 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1921 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1981 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2041 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2101 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2161 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2221 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2281 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2341 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2401 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2461 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2521 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2581 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2641 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2701 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2761 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2821 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2881 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2941 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3001 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3061 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3361 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3421 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cac
//

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