pET-11b - 91蜜桃视频

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0308	pET-11b	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	T7/lac
复制子:	ColE1 ori
终止子:	T7 terminator
质粒分类:	大肠杆菌载体；笔贰罢系列表达质粒
质粒大小:	5676bp
质粒标签:	N-T7
原核抗性:	氨苄青霉素础尘辫
克隆菌株:	顿贬5α
培养条件:	37℃，有氧，尝叠
表达宿主:	BL21(DE3)
培养条件:	37℃，有氧，尝叠
诱导方式:	滨笔罢骋或乳糖及其类似物
5'测序引物:	T7:TAATACGACTCACTATAGGG
3'测序引物:	T7-ter:TGCTAGTTATTGCTCAGCGG

质粒介绍

pET-11a-d 载体具有N端T7标签和 BamH I 多克隆克隆位点。这些载体是很多pET家族载体的前身。pET-21a-d( )系列载体分别对应于 pET-11a-d 载体，但是包含有几个额外的特征序列。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。

质粒图谱

质粒序列

LOCUS       pET 11b(+)    5676 bp     DNA circular   SYN
DEFINITION pET11b
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.miaolingbio.com/
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="pET11b"
                     /mol_type="other DNA"
     terminator      complement(188..316)
                     /label="T7_terminator"
     misc_feature    256..274
                     /label="T7_Terminal_primer"
     misc_feature    complement(327..359)
                     /label="T7_leader"
     misc_feature    complement(342..360)
                     /label="Xpress_fwd_primer"
     misc_feature    complement(368..384)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(402..429)
                     /label="lacO"
     promoter        complement(429..447)
                     /label="T7_promoter"
     misc_feature    complement(531..550)
                     /label="pBRrevBam_primer"
     CDS             584..1084
                     /label="ORF frame 2"
     misc_feature    825..1916
                     /label="lacI"
     CDS             957..1916
                     /label="ORF frame 3"
     CDS             complement(1699..2394)
                     /label="ORF frame 1"
     CDS             1972..2589
                     /label="ORF frame 1"
     gene            1975..2589
                     /label="tet (576 - 1190)"
                     /gene="tet (576 - 1190)"
     misc_feature    3228..3419
                     /label="ROP"
     misc_feature    complement(3413..3435)
                     /label="pGEX_3_primer"
     gene            complement(4608..5468)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(4608..5468)
                     /label="ORF frame 2"
     promoter        complement(5510..5538)
                     /label="AmpR_promoter"
ORIGIN
    1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
   61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atcccgaccc atttgctgtc caccagtcat gctagccata
361 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
421 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc
481 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
541 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
601 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
661 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
721 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct
781 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac
841 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
901 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
961 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
1021 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
1081 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
1141 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
1201 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
1261 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
1321 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
1381 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
1441 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
1501 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
1561 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
1621 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata
1681 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
1741 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
1801 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
1861 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
1921 aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg
1981 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
2041 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
2101 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
2161 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
2221 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
2281 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
2341 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
2401 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc
2461 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
2521 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
2581 tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag
2641 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca
2701 tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc
2761 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
2821 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
2881 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
2941 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
3001 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
3061 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
3121 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
3181 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
3241 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
3301 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
3361 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
3421 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
3481 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
3541 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
3601 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
3661 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
3721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
3781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
3841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
3901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
3961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
4021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
4081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
4141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
4201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
4261 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
4321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
4381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
4441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
4501 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
4561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
4621 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
4681 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
4741 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
4801 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
4861 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
4921 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
4981 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
5041 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
5101 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
5161 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
5221 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
5281 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
5341 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
5401 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
5461 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
5521 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
5581 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
5641 aataggcgta tcacgaggcc ctttcgtctt caagaa
//