pET-5a

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0305	pET-5a	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	T7
复制子:	ColE1 ori
终止子:	T7 terminator
质粒分类:	大肠杆菌载体；笔贰罢系列表达质粒
质粒大小:	4134bp
质粒标签:	N-T7
原核抗性:	氨苄青霉素础尘辫
克隆菌株:	顿贬5α
培养条件:	37℃，有氧，尝叠
表达宿主:	BL21(DE3)
培养条件:	37℃，有氧，尝叠
诱导方式:	无需诱导
5'测序引物:	T7:TAATACGACTCACTATAGGG
3'测序引物:	T7-ter:TGCTAGTTATTGCTCAGCGG
备注:	组成型表达

质粒介绍

pET-5a是一个组成型表达质粒，无需诱导即可在BL21(DE3)中表达，大小为4134bp，含有氨苄青霉素抗性基因，能够在Amp的板子上生长。

质粒图谱

质粒序列

LOCUS       Exported                4134 bp ds-DNA     circular SYN 25-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pET-5a
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 4134)
AUTHORS   .
TITLE     Direct Submission
JOURNAL   Exported Thursday, August 25, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(13..45)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             53..75
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(107..125)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1686..1877
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1979..2121
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2307..2895)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3066..3926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3927..4031)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ttcggatccg cgacccattt gctgtccacc agtcatgcta gccatatgta tatctccttc
       61 ttaaagttaa acaaaattat ttctagaggg aaaccgttgt ggtctcccta tagtgagtcg
      121 tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
      181 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      241 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      301 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
      361 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      421 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
      481 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
      541 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
      601 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
      661 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
      721 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
      781 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
      841 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
      901 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
      961 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     1021 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca
     1081 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc
     1141 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg
     1201 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     1261 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     1321 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
     1381 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
     1441 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc
     1501 gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt
     1561 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca
     1621 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg
     1681 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     1741 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
     1801 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg
     1861 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     1921 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     1981 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     2041 agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta
     2101 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2161 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2221 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2281 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2341 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2401 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2461 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2521 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2581 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2641 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2701 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2761 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2821 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2881 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2941 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3061 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3121 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3181 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3241 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3301 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3361 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3421 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3481 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3541 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3601 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3661 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     3721 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3781 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3841 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3901 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3961 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4021 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     4081 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa
//

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