pQE-TriSystem His-Strep2 - 91蜜桃视频

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0649	pQE-TriSystem His-Strep2	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	p10
复制子:	ColE1 ori
终止子:	T7 terminator
质粒分类:	大肠杆菌载体；辫蚕贰系列表达质粒
质粒大小:	5796bp
原核抗性:	氨苄青霉素础尘辫
克隆菌株:	顿贬5α
培养条件:	37℃，有氧，尝叠
表达宿主:	M15
培养条件:	37℃，有氧，尝叠
诱导方式:	滨笔罢骋或乳糖及其类似物

质粒介绍

质粒图谱

质粒序列

LOCUS       Exported                5796 bp ds-DNA     circular SYN 05-MAY-2014
DEFINITION Vector for expression in bacterial, insect, and vertebrate cells of
            proteins with an N-terminal Strep-Tag II tag and a C-terminal 8xHis
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-TriSystem His-Strep2
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 5796)
AUTHORS   Qiagen
TITLE     Direct Submission
JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        62..367
                     /note="CMV enhancer"
                     /note="human cytomegalovirus (CMV) immediate early
                     enhancer"
     promoter        373..650
                     /note="chicken beta-actin promoter"
     exon            651..742
                     /note="Exon 1"
     intron          743..1004
     exon            1005..1649
                     /note="Exon 2"
     promoter        1073..1116
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    1092..1108
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1143..1252
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     RBS             1255..1260
                     /note="ribosome binding site"
     CDS             1267..1269
                     /codon_start=1
                     /note="ATG"
                     /note="start codon"
                     /translation="M"
     CDS             1279..1302
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin, an engineered
                     form of streptavidin"
                     /note="Strep-Tag II"
                     /translation="WSHPQFEK"
     misc_feature    1307..1446
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1408..1440
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /note="HSV tag"
                     /translation="QPELAPEDPED"
     CDS             1447..1470
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /note="8xHis"
                     /translation="HHHHHHHH"
     misc_feature    1471..1481
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1625..1630
     terminator      1647..1758
                     /note="rabbit globin terminator"
     terminator      1768..1815
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     misc_recomb     1828..3286
                     /note="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     CDS             complement(1945..3286)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /note="ORF1629"
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      complement(3445..4032)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4045..4905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_recomb     4971..2
                     /note="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     CDS             4971..5095
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /note="lef2"
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5133..5738)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /note="ORF603"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 ctctagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
       61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      361 taccatgcat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      421 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      481 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      541 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      601 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgtgc
      661 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      721 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctt cgggctgtaa
      781 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      841 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      961 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1021 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgga aatcataaaa
     1081 aattatttgc tttgtgagcg gataacaatt ataatagatt caattgaggc ctcgaccacc
     1141 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     1201 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
     1261 tataccatgg cgagcgcgtg gagccacccg cagttcgaaa aaatatcccg ggagctcgtg
     1321 gatccgaatt ctcagatctc ggcgcgcctg caggtcgacg gtaccggttc gaagcttgcg
     1381 gccgcacagc tgtatacacg tgcaagccag ccagaactcg ccccggaaga ccccgaggat
     1441 ctcgagcacc accatcacca tcaccatcac taagtgatta acctcaggtg caggctgcct
     1501 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat
     1561 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     1621 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     1681 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagtt tttggtttag
     1741 agtttggcaa catatgccca tatgtaacta gcataacccc ttggggcctc taaacgggtc
     1801 ttgaggggtt ttttgctgaa agcatgcgga ggaaattctc cttgaagttt ccctggtgtt
     1861 caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt attaaaacac
     1921 gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga
     1981 caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag
     2041 agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat
     2101 agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc
     2161 tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa
     2221 tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa
     2281 aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt
     2341 aaacacgtta aatagagctt ggacatattt aacatcgggc gtgttagctt tattaggccg
     2401 attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat
     2461 agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga
     2521 gctttttgga attatttctg attgcgggcg tttttgggcg ggtttcaatc taactgtgcc
     2581 cgattttaat tcagacaaca cgttagaaag cgatggtgca ggcggtggta acatttcaga
     2641 cggcaaatct actaatggcg gcggtggtgg agctgatgat aaatctacca tcggtggagg
     2701 cgcaggcggg gctggcggcg gaggcggagg cggaggtggt ggcggtgatg cagacggcgg
     2761 tttaggctca aatgtctctt taggcaacac agtcggcacc tcaactattg tactggtttc
     2821 gggcgccgtt tttggtttga ccggtctgag acgagtgcga tttttttcgt ttctaatagc
     2881 ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg
     2941 tggagcgggc ggcaattcag acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt
     3001 aggcacggga gaaggtggtg gcggcggtgc cgccggtata atttgttctg gtttagtttg
     3061 ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct
     3121 gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta taattggaat acaaatcgta
     3181 aaaatctgct ataagcattg taatttcgct atcgtttacc gtgccgatat ttaacaaccg
     3241 ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag ctcggaacgc tgcgctcggt
     3301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga gcatcacaaa
     3481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3841 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4021 aaaggatctc aagaagatcc tttgttacca atgcttaatc agtgaggcac ctatctcagc
     4081 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4141 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4201 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4261 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4321 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4381 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4441 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4501 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4561 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4621 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4681 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4741 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4801 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4861 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4921 atattattga agcatttatc agggttattg tctcatgtcc gcgcgtttcc tgcatctttt
     4981 aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa ctgtcgacaa
     5041 gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt attgaataat
     5101 aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa cgcaacaaga
     5161 acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag gtaatattta
     5221 aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt ttcacataaa
     5281 ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt ctcttcataa
     5341 aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt aaattttttg
     5401 ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg catagttttt
     5461 ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc tttaattatt
     5521 aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt gccggcatag
     5581 tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt aacataactt
     5641 tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc tatactattg
     5701 tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg cacaaactaa
     5761 tatcacaaac tggaaatgtc tatcaatata tagttg
//