pQE-T7(cloning region) - 91蜜桃视频

产物信息
基本信息
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0643	pQE-T7(cloning region)	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	T7
终止子:	T7 terminator
质粒分类:	大肠杆菌载体；辫蚕贰系列表达质粒
质粒大小:	276bp
原核抗性:	氨苄青霉素础尘辫
克隆菌株:	顿贬5α
培养条件:	37℃，有氧，尝叠
表达宿主:	M15
培养条件:	37℃，有氧，尝叠
诱导方式:	滨笔罢骋或乳糖及其类似物

质粒介绍

质粒序列

LOCUS       Exported                 276 bp ds-DNA     linear   SYN 10-SEP-2012
DEFINITION Cloning region of pQE-T7, the parental vector for the QIAgenes
            system.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-T7 (cloning region)
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 276)
AUTHORS   Qiagen
TITLE     Direct Submission
JOURNAL   Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
COMMENT     The 6xHis tag can be removed by cutting with NdeI, purifying the cut
            vector, and religating.

            The QIAgene can be excised with NdeI and XhoI.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        16..34
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    35..59
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             104..106
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             110..127
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             128..130
                     /codon_start=1
                     /note="QIAgene start codon"
                     /translation="M"
     misc_feature    131..136
                     /note="stop codons"
                     /note="two tandem stop codons"
     CDS             145..162
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      229..276
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
ORIGIN
        1 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
       61 ctctagaaat aattttgttt aactttaaga aggagatata catatgaaac accatcacca
      121 tcaccatatg tagtaagact cgagcaccac caccaccacc actgagatcc ggctgctaac
      181 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      241 cttggggcct ctaaacgggt cttgaggggt tttttg
//