LOCUS Exported 3618 bp ds-DNA circular SYN 22-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3618)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3618
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..38
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 164..181
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 185..217
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 221..244
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress(TM) tag
/translation="DLYDDDDK"
CDS 251..952
/codon_start=1
/product="monomeric cyan-excitable red fluorescent protein
(Laviv et al., 2016)"
/label=mCyRFP1
/translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTA
RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
VLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
KKLKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
SNLGGGMDELYK"
terminator 1033..1080
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1177..1632
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1658..1762
/gene="bla"
/label=AmpR promoter
CDS 1763..2623
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2794..3382
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
61 agaatcaccg agtttattct tgacacctga tgcgatgaat gatataatag gaaagtactg
121 ttttgtttaa ctttaagaag gggatataca tatgcggggt tctcatcatc atcatcatca
181 tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga
241 taaggatccg atggtgagca agggcgagga gctgatcaag gagaacatga gaagcaagct
301 gtacctggaa ggcagcgtga acggccacca gttcaagtgc acccacgaag gggagggcaa
361 gccctacgag ggcaagcaga ccgcgaggat caaggtggtg gagggaggcc ccctgccgtt
421 cgcattcgac atcctggcca ccatgtttat gtacgggagc aaggtgttca tcaagtaccc
481 cgccgacctc cccgattatt ttaagcagtc cttccctgag ggcttcacat gggagagagt
541 catggtgttc gaagacgggg gcgtgctgac cgccacccag gacaccagcc tccaggacgg
601 cgagctcatc tacaacgtca agctcagagg ggtgaacttc ccagccaacg gccccgtgat
661 gcagaagaaa acactgggct gggagcccag caccgagacc atgtaccccg ctgacggcgg
721 cctggaaggc agatgcgaca agaagctgaa gctcgtgggc gggggccacc tgcacgtcaa
781 cttcaagacc acatacaagt ccaagaaacc cgtgaagatg cccggcgtcc actacgtgga
841 ccgcagactg gaaagaatca aggaggccga caacgagacc tacgtcgagc agtacgagca
901 cgctgtggcc agatactcca acctgggcgg aggcatggac gagctgtaca agtaatgaat
961 tcgaagcttg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
1021 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
1081 ctgaaaggag gaactatatc cggatctggc gtaatagcga agaggcccgc accgatcgcc
1141 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
1201 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
1261 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
1321 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
1381 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
1441 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
1501 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
1561 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
1621 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
1681 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
1741 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
1801 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
1861 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
1921 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
1981 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
2041 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
2101 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
2161 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
2221 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
2281 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
2341 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
2401 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
2461 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
2521 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
2581 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
2641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
2701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
2761 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
2821 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
2881 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
2941 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
3001 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
3061 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
3121 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
3181 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
3241 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
3301 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
3361 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
3421 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
3481 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
3541 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
3601 gccgattcat taatgcag
//