pRSET-BFP

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0917	pRSET-BFP	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	T7
复制子:	pUC ori，F1 ori
终止子:	T7 terminator
质粒分类:	大肠杆菌载体；辫搁厂贰罢系列表达质粒
质粒大小:	3600bp
质粒标签:	狈-贬颈蝉，狈-贰碍，狈-齿辫谤别蝉蝉
原核抗性:	氨苄青霉素础尘辫
克隆菌株:	顿贬5α
培养条件:	37℃，有氧，尝叠
表达宿主:	叠尝21(顿贰3)系列，推荐叠尝21厂迟补谤（顿贰3)
培养条件:	37℃，有氧，尝叠
诱导方式:	滨笔罢骋或乳糖及其类似物
5'测序引物:	T7 Forward: 5-TAATACGACTCACTATAGGG-3
3'测序引物:	根据序列设计
备注:	辫搁厂贰罢表达质粒

质粒介绍

质粒图谱

质粒序列

LOCUS       Exported                3600 bp ds-DNA     circular SYN 29-七月-2016
DEFINITION synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRSET-BFP
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 3600)
AUTHORS   .
TITLE     Direct Submission
JOURNAL   Exported 2016年7月29日 from SnapGene Viewer 2.8.1
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        9..27
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             101..118
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             122..154
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             158..181
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /note="Xpress(TM) tag"
                     /translation="DLYDDDDK"
     CDS             209..928
                     /codon_start=1
                     /product="blue variant of GFP"
                     /note="BFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      1004..1051
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1148..1603
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1629..1733
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1734..2594
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2765..3353
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gcggggttct catcatcatc atcatcatgg
      121 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
      181 ggatcgatgg ggatccgaat tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
      241 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      301 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      361 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgagccacg gcgtccagtg
      421 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      481 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      541 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      601 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacgt
      661 ctatatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa
      721 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      781 cggccccgtg ctgctgcccg acagccacta cctgagcacc cagtccgccc tgagcaaaga
      841 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      901 tctcggcatg gacgagctgt acaagtaact cgagaagctt gatccggctg ctaacaaagc
      961 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1021 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatctgg
     1081 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1141 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1201 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     1261 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1321 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1381 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1441 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1501 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1561 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     1621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1981 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2041 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2101 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2161 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2221 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2281 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2341 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2401 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2461 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2521 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2581 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2881 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3421 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3481 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3541 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagg atctcgatcc
//

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