pKJE7

产物信息
基本信息
质粒介绍
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0487	pKJE7	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

复制子:	p15A ori
质粒分类:	大肠杆菌载体；伴侣分子表达质粒
质粒大小:	7.2kb
原核抗性:	氯霉素颁丑濒
克隆菌株:	顿贬5α
培养条件:	37℃，有氧，尝叠
表达宿主:	大肠杆菌
培养条件:	37℃，有氧，尝叠
诱导方式:	阿拉伯糖
备注:	伴侣分子表达质粒

质粒图谱

质粒序列

LOCUS       Exported                7193 bp ds-DNA     circular SYN 12-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 14
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 7193)
AUTHORS   .
TITLE     Direct Submission
JOURNAL   Exported Monday, September 12, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      5802..6346
                     /direction=RIGHT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        6872..6974
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             6975..441
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaag aatagttacg
      541 gcttatgaca tctttgtgga cacatcattc actttttatt cacatccggc cctgaactcg
      601 ctaggacttg ccccggtgca ttttttaaat acccgcgaaa aatagagctg atcgtcaaat
      661 ccaacattgc gcccaacggt cgctatcggc attcgcgtag tgctaagcag aagtttcgcc
      721 tggctgatac gctgatcttc gcgccagctc aatacgctaa tgcctaactg ctggcggaac
      781 agatgtgata accgggaggg cgacaggcag acatgctggg cgacgctggc gatatcaaaa
      841 tggctgtccg ccagatggtc gctgatatac tggcaggcat cgcgcacacg gctatccatc
      901 ggcgggtgca acgactcatt aattaccgcc atacgtctga gcaacaactg ctccagcaga
      961 ttgatcgcca gtagctcaga atagcgacct tccccttgcc cggcgctgat gatctgcccg
     1021 aacagttcgc tgaaatgcgg ctggcgcgcc tcgtccgggc ggaaaaatcc tgtctgggca
     1081 aagattgtcg gccaggtcag ccactcctgc cagtaggcgc gaggccggaa ataaacccac
     1141 tggtgatacc actcgctggc gtccggatgc cgtccatagt gatgaatctc gcccggcgga
     1201 aacaataata tatcgccagg ccgacagaca aactgctcgc cattattatt aatgacgccc
     1261 tctccgcgga tggtcaggtt aagaatatat cccttcatgc ccaacggacg atcgataaaa
     1321 aaatccagat atccattcgc ttcaattggc gtcagcccgg cgaccagatg ggcattaaat
     1381 gaatatcccg gcaatagcgg atcattttgc gtttcagcca tgatttctct accccccgat
     1441 gttcagagaa gaaccaaatt gtccatatcg accaggacga cagagcttcc gtctccgcaa
     1501 gactttgcgc ttgatgaaag cacgtatcaa ccccgcttgt gaaaagcgct ttgtaacaaa
     1561 agcgtacagt tcaggcgata aaattaagta acagaagtgt ctataactat ggctggaatg
     1621 tccacattga atatttgcac agcgtcacac tttgcaaagc attagcattt ttgtccataa
     1681 gattagcgga tcctgcctga cggtttttgc cgcgactctc tataatttct ccatacctgt
     1741 ttttctggat ggagtaagac catggctatg gattgtggag acgtttagat gggtaaaata
     1801 attggtatcg acctgggtac taccaactct tgtgtagcga ttatggatgg caccactcct
     1861 cgcgtgctgg agaacgccga aggcgatcgc accacgcctt ctatcattgc ctatacccag
     1921 gatggtgaaa ctctagttgg tcagccggct aaacgtcagg cagtgacgaa cccgcaaaac
     1981 actctgtttg cgattaaacg cctgattggt cgccgcttcc aggacgaaga agtacagcgt
     2041 gatgtttcca tcatgccgtt caaaattatt gctgctgata acggcgacgc atgggtcgaa
     2101 gttaaaggcc agaaaatggc accgccgcag atttctgctg aagtgctgaa aaaaatgaag
     2161 aaaaccgctg aagattacct gggtgaaccg gtaactgaag ctgttatcac cgtaccggca
     2221 tactttaacg atgctcagcg tcaggcaacc aaagacgcag gccgtatcgc tggtctggaa
     2281 gtaaaacgta tcatcaacga accgaccgca gctgcgctgg cttacggtct ggacaaaggc
     2341 actggcaacc gtactatcgc ggtttatgac ctgggtggtg gtactttcga tatttctatt
     2401 atcgaaatcg acgaagttga cggcgaaaaa accttcgaag ttctggcaac caacggtgat
     2461 acccacctgg ggggtgaaga cttcgacagc cgtctgatca actatctggt tgaagaattc
     2521 aagaaagatc agggcattga cctgcgcaac gatccgctgg caatgcagcg cctgaaagaa
     2581 gcggcagaaa aagcgaaaat cgaactgtct tccgctcagc agaccgacgt taacctgcca
     2641 tacatcactg cagacgcgac cggtccgaaa cacatgaaca tcaaagtgac tcgtgcgaaa
     2701 ctggaaagcc tggttgaaga tctggtaaac cgttccattg agccgctgaa agttgcactg
     2761 caggacgctg gcctgtccgt atctgatatc gacgacgtta tcctcgttgg tggtcagact
     2821 cgtatgccaa tggttcagaa gaaagttgct gagttctttg gtaaagagcc gcgtaaagac
     2881 gttaacccgg acgaagctgt agcaatcggt gctgctgttc agggtggtgt tctgactggt
     2941 gacgtaaaag acgtactgct gctggacgtt accccgctgt ctctgggtat cgaaaccatg
     3001 ggcggtgtga tgacgacgct gatcgcgaaa aacaccacta tcccgaccaa gcacagccag
     3061 gtgttctcta ccgctgaaga caaccagtct gcggtaacca tccatgtgct gcagggtgaa
     3121 cgtaaacgtg cggctgataa caaatctctg ggtcagttca acctagatgg tatcaacccg
     3181 gcaccgcgcg gcatgccgca gatcgaagtt accttcgata tcgatgctga cggtatcctg
     3241 cacgtttccg cgaaagataa aaacagcggt aaagagcaga agatcaccat caaggcttct
     3301 tctggtctga acgaagatga aatccagaaa atggtacgcg acgcagaagc taacgccgaa
     3361 gctgaccgta agtttgaaga gctggtacag actcgcaacc agggcgacca tctgctgcac
     3421 agcacccgta agcaggttga agaagcaggc gacaaactgc cggctgacga caaaactgct
     3481 atcgagtctg cgctgactgc actggaaact gctctgaaag gtgaagacaa agccgctatc
     3541 gaagcgaaaa tgcaggaact ggcacaggtt tcccagaaac tgatggaaat cgcccagcag
     3601 caacatgccc agcagcagac tgccggtgct gatgcttctg caaacaacgc gaaagatgac
     3661 gatgttgtcg acgctgaatt tgaagaagtc aaagacaaaa aataatcgcc ctataaacgg
     3721 gtaattatac tgacacgggc gaaggggaat ttcctccccg cccgtgcatt catctagggg
     3781 caatttaaaa aagatggcta agcaagatta ttacgagatt ttaggcgttt ccaaaacagc
     3841 ggaagagcgt gaaatcagaa aggcctacaa acgcctggcc atgaaatacc acccggaccg
     3901 taaccagggt gacaaagagg ccgaggcgaa atttaaagag atcaaggaag cttatgaagt
     3961 tctgaccgac tcgcaaaaac gtgcggcata cgatcagtat ggtcatgctg cgtttgagca
     4021 aggtggcatg ggcggcggcg gttctggcgg cggcgcagac ttcagcgata tttttggtga
     4081 cgttttcggc gatatttttg gcggcggacg tggtcgtcaa cgtgcggcgc gcggtgctga
     4141 tttacgctat aacatggagc tcaccctcga agaagctgta cgtggcgtga ccaaagagat
     4201 ccgcattccg actctggaag agtgtgacgt ttgccacggt agcggtgcaa aaccaggtac
     4261 acagccgcag acttgtccga cctgtcatgg ttctggtcag gtgcagatgc gccagggatt
     4321 cttcgctgta cagcagacct gtccacactg tcagggccgc ggtacgctga tcaaagatcc
     4381 gtgcaacaaa tgtcatggtc atggtcgtgt tgagcgcagc aaaacgctgt ccgttaaaat
     4441 cccggcaggg gtggacactg gagaccgcat ccgtcttgcg ggcgaaggtg aagcgggcga
     4501 gcatggcgca ccggcaggcg atctgtacgt tcaggttcag gttaaacagc acccgatttt
     4561 cgagcgtgaa ggcaacaacc tgtattgcga agtcccgatc aacttcgcta tggcggcgct
     4621 gggtggcgaa atcgaagtac cgacccttga tggtcgcgtc aaactgaaag tgcctggcga
     4681 aacccagacc ggtaagctat tccgtatgcg cggtaaaggc gtcaagtctg tccgcggtgg
     4741 cgcgcagggt gatttgctgt gccgcgttgt cgtcgaaaca ccggtaggcc tgaacgaaag
     4801 gcagaaacag ctgctgcaag agctgcaaga aagcttcggt ggcccaaccg gcgagcacaa
     4861 cagcccgcgc tcaaagagct tctttgatgg tgtgaagaag ttttttgacg acctgacccg
     4921 ctaacctcgc ggagaaattc atgagtagta aagaacagaa aacgcctgag gggcaagccc
     4981 cggaagaaat tatcatggat cagcacgaag agattgaggc agttgagcca gaagcttctg
     5041 ctgagcaggt ggatccgcgc gatgaaaaag ttgcgaatct cgaagctcag ctggctgaag
     5101 cccagacccg tgaacgtgac ggcattttgc gtgtaaaagc cgaaatggaa aacctgcgtc
     5161 gtcgtactga actggatatt gaaaaagccc acaaattcgc gctggagaaa ttcatcaacg
     5221 aattgctgcc ggtgattgat agcctggatc gtgcgctgga agtggctgat aaagctaacc
     5281 cggatatgtc tgcgatggtt gaaggcattg agctgacgct gaagtcgatg ctggatgttg
     5341 tgcgtaagtt tggcgttgaa gtgatcgccg aaactaacgt cccactggac ccgaatgtgc
     5401 atcaggccat cgcaatggtg gaatctgatg acgttgcgcc aggtaacgta ctgggcatta
     5461 tgcagaaggg ttatacgctg aatggtcgta cgattcgtgc ggcgatggtt actgtagcga
     5521 aagcaaaagc ttaatttctg ctttcgtaat aattcacggc cctgcatgca agctcagatc
     5581 tgagcttggc tgttttggcg gatgagagaa gaggtcgact ctagaggatc tactagtcat
     5641 aggcgccgat atcagatccg gtaccaagct tatcgatgat aagctgtcaa acatgagaat
     5701 tacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact
     5761 gaaatctaga aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg
     5821 cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt
     5881 ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa
     5941 cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca
     6001 attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg
     6061 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
     6121 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
     6181 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     6241 cgccagggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga
     6301 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc
     6361 ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt
     6421 tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
     6481 gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc
     6541 ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac
     6601 actccgctag cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc
     6661 tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca
     6721 tacactaaat cagtaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg
     6781 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
     6841 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
     6901 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     6961 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     7021 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     7081 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     7141 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttc
//