pBV220

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY0367	pBV220	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	pR/pL
复制子:	pUC ori
质粒分类:	大肠杆菌载体；蛋白表达质粒
质粒大小:	3666bp
原核抗性:	氨苄青霉素础尘辫
克隆菌株:	顿贬5α
培养条件:	37℃，有氧，尝叠
表达宿主:	大肠杆菌
培养条件:	37℃，有氧，尝叠
5'测序引物:	pBV220-F: 5′-AAGAAGGGCAGCATTCAAAG-3‘
3'测序引物:	pBV220-R: 5′-CTGCGTTCTGATTTAATCTG-3‘
备注:	温控表达质粒

质粒介绍

质粒图谱

质粒序列

LOCUS       Exported File           3666 bp ds-DNA    circular SYN 21-9-2015
DEFINITION .
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 3
SOURCE      synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE   1 (bases 1 to 3666)
AUTHORS   .
TITLE     Direct Submission
JOURNAL   Exported 2015-9-21 from MLCC
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      237..323
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      415..442
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        460..551
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      1584..2172
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gaattcccgg ggatccgtcg acctgcagcc aagcttctgt tttggcttat gagagaagat
       61 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
      121 tcccggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
      181 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gccaactgcc aggcatcaaa
      241 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      301 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      361 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaagg aatcagaagg
      421 ccatcctgac ggatggcctt tttgcgtttc tacaaactct ttgtttattt ttctaaatac
      481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      781 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      961 tgacaacgat cgggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1021 gtaactcgcc ttgatcgttg ggaaccggat ctgaatgaag ccataccaaa cgacgagcgt
     1081 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1141 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1201 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1261 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1321 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1381 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1441 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1501 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1561 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1621 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1681 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     1741 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1801 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1861 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1921 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     1981 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2041 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2101 gtcgggtttc gccaacctct gacttgagcg tcgattttgt gatgctcgtc aggggggcgg
     2161 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2221 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2281 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2341 gaggaagcgg aagagcgccc ttatctttcc ctttattttt gctgcggtaa gtcgcataaa
     2401 aaccattctt cataattcaa tccatttact atgttatgtt ctgaggggag tgaaaattcc
     2461 cctaattcga tgaagattct tgctcaattg ttatcagcta tgcgccgacc agaacacctt
     2521 gccgatcagc caaacgtctc ttcaggccac tgactagcga taactttccc cacaacggaa
     2581 caactctcat tgcatgggat cattgggtac tgtgggttta gtggttgtaa aaacacctga
     2641 ccgctatccc tgatcagttt cttgaaggta aactcatcac ccccaagtct ggctatgcag
     2701 aaatcacctg gctcaacagc ctgctcaggg tcaacgagaa ttaacattcc gtcaggaaag
     2761 cttggcttgg agcctgttgg tgcggtcatg gaattacctt caacctcaag ccagaatgca
     2821 gaatcactgg cttttttggt tgtgcttacc catctctccg catcaccttt ggtaaaggtt
     2881 ctaagcttag gtgagaacat ccctgcctga acatgagaaa aaacagggta ctcatactca
     2941 cttctaagtg acggctgcat actaaccgct tcatacatct cgtagatttc tctggcgatt
     3001 gaagggctaa attcttcaac gctaactttg agaatttttg caagcaatgc ggcgttataa
     3061 gcatttaatg cattgatgcc attaaataaa gcaccaacgc ctgactgccc catccccatc
     3121 ttgtctgcga cagattcctg ggataagcca agttcatttt tctttttttc ataaattgct
     3181 ttaaggcgac gtgcgtcctc aagctgctct tgtgttaatg gtttcttttt tgtgctcata
     3241 cgttaaatct atcaccgcaa gggataaata tctaacaccg tgcgtgttga ctattttacc
     3301 tctggcggtg ataatggttg catgtactaa ggaggttgta tggaacaacg cataaccctg
     3361 aaagattatg caatgcgctt tgggcaaacc aagacagcta aaagatctct cacctaccaa
     3421 acaatgcccc cctgcaaaaa ataaattcat ataaaaaaca tacagataac catctgcggt
     3481 gataaattat ctctggcggt gttgacataa ataccactgg cggtgatact gagcacatca
     3541 gcaggacgca ctgaccacca tgaaggtgac gctcttaaaa attaagccct gaagaagggc
     3601 agcattcaaa gcagaaggct ttggggtgtg tgatacgaaa cgaagcattg gttaaaaatt
     3661 aaggag
//

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