LOCUS Exported 6116 bp ds-DNA circular SYN 13-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6116
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 5..384
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 386..661
/label=chicken beta-actin promoter
intron 662..1671
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
misc_feature 1768..2354
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 2355..3074
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_site 3203..3258
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
promoter 3792..3987
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3838..3973
/label=SV40 ori
/note="SV40 origin of replication"
polyA_signal 3993..4127
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4366..4954)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5125..5985)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5986..6090)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1501 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
1561 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
1621 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
1681 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcctcga ggatatcgcg
1741 gccgctacgt aaattccgcc cccccccccc ctctccctcc ccccccccta acgttactgg
1801 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
1861 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
1921 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
1981 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
2041 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
2101 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
2161 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
2221 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
2281 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
2341 atatggccac aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
2401 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
2461 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
2521 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
2581 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
2641 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
2701 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
2761 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
2821 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
2881 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
2941 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
3001 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
3061 agctgtacaa gtaaagcggc cggccgccag cacagtggtc gatcgaccaa tgccctggct
3121 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
3181 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
3241 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
3301 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
3361 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
3421 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
3481 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
3541 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc
3601 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
3661 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
3721 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
3781 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
3841 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
3901 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
3961 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
4021 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
4081 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
4141 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
4201 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
4261 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
4321 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
4381 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
4441 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
4501 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
4561 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
4621 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
4681 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
4741 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
4801 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
4861 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
4921 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
4981 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
5041 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
5101 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
5161 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
5221 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
5281 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
5341 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
5401 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
5461 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
5521 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
5581 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
5641 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
5701 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
5761 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
5821 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
5881 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
5941 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
6001 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
6061 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//