pHcRed1-C1

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY1393	pHcRed1-C1	100μ濒菌种冻干粉	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	CMV IE promoter
复制子:	pUC ori，F1 ori，SV40 ori
终止子:	SV40 early polyA signals
质粒分类:	哺乳细胞质粒；哺乳荧光质粒；哺乳红色质粒
质粒大小:	4698bp
质粒标签:	N-HcRed1
原核抗性:	卡那霉素碍补苍（50μ驳/μ濒）
筛选标记:	新霉素狈别辞/骋418
克隆菌株:	顿贬5α等大肠杆菌
培养条件:	37℃，有氧，尝叠
表达宿主:	293罢等哺乳细胞
培养条件:	37℃，5%颁翱2
诱导方式:	无需诱导，组成型表达
5'测序引物:	颁惭痴-贵：颁骋颁础础础罢骋骋骋颁骋骋罢础骋骋颁骋罢骋
3'测序引物:	厂惫40-辫辞濒测础-搁：骋础础础罢罢罢骋罢骋础罢骋颁罢础罢罢骋颁
备注:	高拷贝

质粒介绍

pHcRed1-C1质粒是一个哺乳细胞红色荧光蛋白表达载体，CMV IE启动子驱动HcRed1红色荧光蛋白基因和目的基因融合表达。
pHcRed1-C1 is a mammalian expression vector designed to express a protein of interest fused to the C-terminus of the far-red fluorescent protein HcRed1. pHcRed1-C1 can be used to monitor gene expression and protein localization in vivo or as a cotransfection marker; the unmodified vector will express HcRed1 in mammalian cells. HcRed1 was generated by mutagenesis of a non-fluorescent chromoprotein from the reef coral Heteractis crispa . The coding sequence for HcRed1 is human codon-optimized for higher expression in mammalian cells . Genes cloned into the multiple cloning site (MCS) downstream of the HcRed1 coding sequence are expressed as fusions to the C-terminus of HcRed1. The MCS in pHcRed1-C1 is positioned between the HcRed1 coding sequence and the SV40 polyadenylation signal (SV40 poly A). Genes cloned into the MCS will be expressed as fusions to the C-terminus of HcRed1 if they are in the same reading frame as HcRed1 and there are no intervening stop codons. A Kozak consensus sequence upstream of HcRed1 increases the translation efficiency in eukaryotic cells . SV40 poly A signals downstream of the MCS direct proper processing of the 3' end of mRNA transcripts. The vector also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin resistance cassette (Neor)—consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene—allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. pHcRed1-C1 can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 .

质粒图谱

质粒序列

LOCUS Exported 4698 bp ds-DNA circular SYN 22-AUG-2017

DEFINITION synthetic circular DNA

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 4698)

TITLE Direct Submission

JOURNAL Exported Aug 25, 2017 from MiaoLingPlasmid

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..4698

/organism="synthetic DNA construct"

/mol_type="other DNA"

enhancer 61..364

/label=CMV enhancer

/note="human cytomegalovirus immediate early enhancer"

promoter 365..568

/label=CMV promoter

/note="human cytomegalovirus (CMV) immediate early

promoter"

CDS 613..1296

/codon_start=1

/product="far-red fluorescent variant of Heteractis crispa

chromoprotein"

/label=HcRed1

/note="mammalian codon-optimized"

/translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH

VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT

AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL

KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD

LPEKAN"

misc_feature 1297..1362

/label=MCS

/note="multiple cloning site"

polyA_signal 1486..1607

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

rep_origin complement(1614..2069)

/direction=LEFT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 2096..2200

/gene="bla"

/label=AmpR promoter

promoter 2202..2559

/label=SV40 promoter

/note="SV40 enhancer and early promoter"

rep_origin 2410..2545

/label=SV40 ori

/note="SV40 origin of replication"

CDS 2594..3388

/codon_start=1

/gene="aph(3')-II (or nptII)"

/product="aminoglycoside phosphotransferase from Tn5"

/label=NeoR/KanR

/note="confers resistance to neomycin, kanamycin, and G418

(Geneticin(R))"

/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

polyA_signal 3620..3667

/label=HSV TK poly(A) signal

/note="herpes simplex virus thymidine kinase

polyadenylation signal (Cole and Stacy, 1985)"

rep_origin 3996..4584

/direction=RIGHT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

601 ccggtcgcca ccatggtgag cggcctgctg aaggagagta tgcgcatcaa gatgtacatg

661 gagggcaccg tgaacggcca ctacttcaag tgcgagggcg agggcgacgg caaccccttc

721 gccggcaccc agagcatgag aatccacgtg accgagggcg cccccctgcc cttcgccttc

781 gacatcctgg ccccctgctg cgagtacggc agcaggacct tcgtgcacca caccgccgag

841 atccccgact tcttcaagca gagcttcccc gagggcttca cctgggagag aaccaccacc

901 tacgaggacg gcggcatcct gaccgcccac caggacacca gcctggaggg caactgcctg

961 atctacaagg tgaaggtgca cggcaccaac ttccccgccg acggccccgt gatgaagaac

1021 aagagcggcg gctgggagcc cagcaccgag gtggtgtacc ccgagaacgg cgtgctgtgc

1081 ggccggaacg tgatggccct gaaggtgggc gaccggcacc tgatctgcca ccactacacc

1141 agctaccgga gcaagaaggc cgtgcgcgcc ctgaccatgc ccggcttcca cttcaccgac

1201 atccggctcc agatgctgcg gaagaagaag gacgagtact tcgagctgta cgaggccagc

1261 gtggcccggt acagcgacct gcccgagaag gccaactccg gactcagatc tcgagctcaa

1321 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg

1381 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac

1441 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca

1501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt

1561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt

1621 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa

1681 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt

1741 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa

1801 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag

1861 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt

1921 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg

1981 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc

2041 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg

2101 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata

2161 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag

2221 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt

2281 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca

2341 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta

2401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga

2461 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag

2521 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg

2581 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt

2641 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt

2701 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc

2761 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc

2821 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga

2881 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat

2941 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca

3001 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga

3061 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc

3121 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat

3181 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga

3241 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg

3301 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt

3361 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa

3421 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg

3481 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg

3541 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg

3601 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg

3661 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga

3721 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg

3781 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt

3841 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg

3901 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga

3961 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta

4021 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa

4081 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact

4141 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca

4201 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt

4261 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg

4321 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag

4381 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta

4441 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat

4501 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg

4561 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc

4621 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac

4681 cgtattaccg ccatgcat

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