LOCUS Exported 13500 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..13500
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 118..447
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 298..433
/label=SV40 ori
/note="SV40 origin of replication"
promoter 495..542
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 561..935
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=Zeo
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 1065..1186
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1637..2225)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2396..3256)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3257..3361)
/gene="bla"
/label=AmpR promoter
enhancer 3627..4006
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4008..4206
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 4224..4404
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4451..4576
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5069..5302
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 5829..5946
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 5996..6236
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 6282..6300
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_RNA 6352..6370
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
promoter 6457..6668
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 6685..6732
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 6733..10833
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the N863A mutation in the HNH
catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 10888..10908
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 10933..11082
/codon_start=1
/product="tetrameric repeat of the minimal activation
domain of herpes simplex virus VP16 (Beerli et al., 1998)"
/label=VP64
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
CDS 11104..11157
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 11158..11556
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=Blast
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 11581..12169
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 12694..12874
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 12906..13130
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 13176..104
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
61 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
121 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
181 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
241 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
301 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
361 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
421 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
481 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
541 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
601 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
661 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
721 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
781 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
841 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
901 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
961 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
1021 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
1081 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
1141 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
1201 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
1261 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
1321 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
1381 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
1441 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
1501 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
1561 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
1621 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
1681 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
1741 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
1801 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
1861 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
1921 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
1981 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
2041 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
2101 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
2161 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
2221 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
2281 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
2341 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
2401 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
2461 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
2521 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
2581 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
2641 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
2701 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
2761 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
2821 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
2881 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
2941 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
3001 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
3061 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
3121 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
3181 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
3241 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
3301 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
3361 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
3421 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
3481 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
3541 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
3601 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
3661 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
3721 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
3781 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
3841 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
3901 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
3961 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
4021 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
4081 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
4141 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
4201 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
4261 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
4321 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
4381 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
4441 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
4501 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
4561 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
4621 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
4681 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
4741 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
4801 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
4861 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
4921 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
4981 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
5041 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
5101 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
5161 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
5221 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
5281 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
5341 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
5401 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
5461 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
5521 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
5581 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
5641 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
5701 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
5761 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
5821 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
5881 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
5941 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattag ctagcgaggg
6001 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
6061 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
6121 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
6181 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
6241 caccggagac gggataccgt ctctgtttta gagctaggcc aacatgagga tcacccatgt
6301 ctgcagggcc tagcaagtta aaataaggct agtccgttat caacttggcc aacatgagga
6361 tcacccatgt ctgcagggcc aagtggcacc gagtcggtgc tttttttgga tcctaggtct
6421 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
6481 tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg
6541 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
6601 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
6661 gaacacaggc gtacggccac catgaaaagg ccggcggcca cgaaaaaggc cggccaggca
6721 aaaaagaaaa aggacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc
6781 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
6841 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
6901 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
6961 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
7021 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
7081 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
7141 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
7201 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
7261 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
7321 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
7381 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
7441 ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
7501 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
7561 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
7621 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
7681 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
7741 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
7801 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
7861 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
7921 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
7981 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
8041 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
8101 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
8161 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
8221 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
8281 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
8341 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
8401 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
8461 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
8521 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
8581 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
8641 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
8701 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
8761 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
8821 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
8881 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
8941 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
9001 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
9061 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
9121 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
9181 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
9241 gtggaccaca tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
9301 accagaagcg acaaggcccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
9361 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
9421 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
9481 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
9541 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
9601 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
9661 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
9721 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
9781 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
9841 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
9901 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
9961 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
10021 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
10081 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
10141 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
10201 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
10261 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
10321 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
10381 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
10441 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
10501 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
10561 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
10621 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
10681 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
10741 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
10801 acacggatcg acctgtctca gctgggaggc gacagcgctg gaggaggtgg aagcggagga
10861 ggaggaagcg gaggaggagg tagcggacct aagaaaaaga ggaaggtggc ggccgctgga
10921 tccggacggg ctgacgcatt ggacgatttt gatctggata tgctgggaag tgacgccctc
10981 gatgattttg accttgacat gcttggttcg gatgcccttg atgactttga cctcgacatg
11041 ctcggcagtg acgcccttga tgatttcgac ctggacatgc tgattaactg tacaggcagt
11101 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg
11161 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
11221 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
11281 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
11341 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
11401 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
11461 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
11521 gaattgctgc cctctggtta tgtgtgggag ggctaagaat tcgatatcaa gcttatcggt
11581 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
11641 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
11701 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
11761 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
11821 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
11881 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
11941 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
12001 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
12061 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
12121 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
12181 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
12241 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
12301 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
12361 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
12421 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
12481 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
12541 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
12601 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
12661 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
12721 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
12781 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
12841 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
12901 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
12961 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
13021 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
13081 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
13141 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
13201 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
13261 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
13321 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
13381 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
13441 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
//