pNZ8148

产物信息
基本信息
质粒介绍
质粒图谱
质粒序列

产物信息

产物货号	产物名称	产物规格	优惠价
XY1366	pNZ8148	20μ濒	?请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μ濒 ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	PnisA
复制子:	谤别辫础，谤别辫颁
终止子:	T
质粒分类:	乳酸杆菌表达质粒
质粒大小:	3167bp
原核抗性:	氯霉素肠丑濒辞谤补尘辫丑别苍颈肠辞濒
筛选标记:	氯霉素肠丑濒辞谤补尘辫丑别苍颈肠辞濒
克隆菌株:	MC1061,BL21 DE3
培养条件:	37℃，有氧，尝叠
表达宿主:	乳酸杆菌
5'测序引物:	根据序列设计

质粒介绍

The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII.
The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus.
pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).

质粒图谱

质粒序列

LOCUS Exported 3167 bp ds-DNA circular SYN 29-AUG-2016

DEFINITION synthetic circular DNA.

ACCESSION .

VERSION .

KEYWORDS pNZ8148

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 3167)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported 2016-2-19 from MiaoLingBio http://www.miaolingbio.com

REFERENCE 2 (bases 1 to 3167)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Monday, August 29, 2016 from SnapGene Viewer 3.1.4

http://www.snapgene.com

FEATURES Location/Qualifiers

source 1..3167

/organism="synthetic DNA construct"

/mol_type="other DNA"

misc_feature 5..188

/note="Pnis"

misc_feature 393..445

/note="Terminator"

misc_feature 787..996

/note="repC"

misc_feature 1264..1962

/note="repA"

misc_feature 2421..3071

/note="Cm"

ORIGIN

1 agatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa

61 ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg

121 gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa

181 taaattataa ggaggcactc accatgggta ctgcaggcat gcggtaccac tagttctaga

241 gagctcaagc tttctttgaa ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa

301 tggcaattaa acaaattaca gcacgtgttg ctttgattga tagccaaaaa gcagcagttg

361 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag

421 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaat

481 cgtttctgag acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg

541 cgttatcgta gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg

601 ttagattatg aaagccgatg actgaatgaa ataataagcg cagcgtcctt ctatttcggt

661 tggaggaggc tcaagggagt ttgagggaat gaaattccct catgggtttg attttaaaaa

721 ttgcttgcaa ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg

781 gaaaaaaatg gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg

841 ccgagtgcca atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttgagggg

901 tttttcaatc gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt

961 aagtagcttt atttttgttt ttatgattac aaagtgatac actaatttta taaaattatt

1021 tgattggagt tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga

1081 caaaagagca agataaaaaa ttaacagata tggcgaaaca aaaagatttt tcaaaatctg

1141 cggttgcggc gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag

1201 cgaaagctcg cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt

1261 atatcatggc tattaaaaat actaaagcta gaaattttgg atttttatta tatcctgact

1321 caattcctaa tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc

1381 ctttacacga tatggacgaa aaaaaagata aagatacatg gaatagtagt gatgttatac

1441 gaaatggaaa gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg

1501 taacaataga aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc

1561 atgttgagat acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg

1621 acgctattgc taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt

1681 ttgatattga ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac

1741 ttttagatat agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc

1801 gccttagggg agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa

1861 caaactctag cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa

1921 gttatgcaaa ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga

1981 gttatttgct gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga

2041 aagatacaga gaaatggaag ttgaattaag tacaacaata gatttattga gaggagggat

2101 tattgaataa ataaaagccc ccctgacgaa agtcgacggc aatagttacc cttattatca

2161 agataagaaa gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac acaaaagacc

2221 acatttttta atgtggtctt ttattcttca actaaagcac ccattagttc aacaaacgaa

2281 aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat attgactttt

2341 aaaaaaggat tgattctaat gaagaaagca gacaagtaag cctcctaaat tcactttaga

2401 taaaaattta ggaggcatat caaatgaact ttaataaaat tgatttagac aattggaaga

2461 gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata accacagaaa

2521 ttgatattag tgttttatac cgaaacataa aacaagaagg atataaattt taccctgcat

2581 ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact ggttacaata

2641 gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt tttgatggtg

2701 tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa gagttttatg

2761 atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt cccaaaacac

2821 ctatacctga aaatgctttt tctctttcta ttattccttg gacttcattt actgggttta

2881 acttaaatat caataataat agtaattacc ttctacccat tattacagca ggaaaattca

2941 ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat tctgtttgtg

3001 atggttatca tgctggattg tttatgaact ctattcagga attgtcagat aggcctaatg

3061 actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt tttctaatgt

3121 cactaacctg ccccgttagt tgaagaaggt ttttatatta cagctcc

91蜜桃视频